resource source identifier rabbit anti-rad21 abcam ab992  (Danaher Inc)

Journal: bioRxiv

Article Title: Identification of the sequences responsible for maternal H19 -ICR hypermethylation with Beckwith-Wiedemann syndrome-like overgrowth in mice

doi: 10.1101/2024.07.07.602442

Figure Lengend Snippet: ChIP-qPCR analysis of CTS1-4 in H19 -ICR mSOC3/+ (n = 6) and WT (n = 4), H19 -ICR mSOC4/+ (n = 7) and WT (n = 6), and H19 -ICR ΔC3,4/+ (n = 7) and WT (n = 5) embryos from 2 litters at 10.5 dpc, as performed using CTCF (A) and RAD21 (B) antibodies. Error bars indicate standard deviation. P -values are indicated (unpaired two-tailed t -test). ns: not significant.

Article Snippet: Chromatin was immunoprecipitated using the following antibodies: rabbit monoclonal anti-CTCF (#3418; Cell Signaling Technology, Danvers, MA, USA) and rabbit polyclonal anti-Rad21 (ab992; Abcam, Cambridge, UK).

Techniques: Standard Deviation, Two Tailed Test

Journal: Nucleic Acids Research

Article Title: ARID1A regulates DNA repair through chromatin organization and its deficiency triggers DNA damage-mediated anti-tumor immune response

doi: 10.1093/nar/gkae233

Figure Lengend Snippet: ARID1A is required for efficient chromatin loop formation. ( A – C ) 4C–seq normalized read counts at indicated viewpoints as well as differential 4C–seq track (log 2 +DSB/–DSB) in WT (grey) ARID1A-KO (blue) cells collected at indicated time points (T0 and T4). 4C–seq data were smoothed using 10-kb spans. ( D – F ) Box plots showing the differential 4C–seq (log 2 +DSB/–DSB) at indicated viewpoints. Four technical replicates from two independent experiments (biological replicates), data are presented mean ± SD, Student's t test. ( G – I ) Enrichment of γH2AX, RAD21 and CTCF, respectively, at the indicated DSBs in WT and ARID1A-KO cells, measured by ChIP-qPCR. n = 3 independent experiments (biological replicates); data are presented as mean ± SEM, Student's t test. Statistical significance is presented as: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns = not significant.

Article Snippet: Rabbit-anti-RAD21 (Abcam, Cat#ab992).

Techniques: